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KMID : 0380619820140020151
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Korean Journal of Food Science and Technology
1982 Volume.14 No. 2 p.151 ~ p.155
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Production of Fungal Lipids V . Effects of Vitamins , Metabolic Intermediates and Mineral Salts on the Growth and Lipid Accumulation of Mucor plumbeus
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이형춘/Lee, Hyeong Choon
신동화/민병용/유진영/Shin, Dong Hwa/Min, Byong Yong/Yoo, Jin Young
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Abstract
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Effects of vitamins, metabolic intermediates and several inorganic mineral salts on the biomass and lipid accumulation of Mucor plumbeus were investigated after 15 days of incubation at 37℃ under static culture condition. The optimum concentrations of various vitamins were 1 γ/ℓ for biotin, and 0.01 g/ℓ for nicotinic acid, pyridoxine, thiamine and riboflavin. Among them pyridoxine was the most stimulatory. The maximum felt weight and lipid content per 50㎖ medium were 2.82±.014 g and 62.8%. Triglyceride content of neutral lipid produced under this condition was 64.9%. The major fatty acids were oleic acid (50.0%), linoleic (23.8%) and palmitic acid (13.9%). Malonic acid was considered not to be desirable even though it stimulated the biomass and lipid accumulation because triglyceride content was lowered considerably comparing with control. MgSO₄·7H₂O was the most stimulatory among the various magnesium salts and its optimum concentration was 5 g/ℓ. Mucor plumbeus did not require NaH₂PO₄ for the stimulation of felt and lipid production. However, the addition of MnCl₂ at the concentration of 2 g/ℓ was stimulatory to show 2.76±0.28g of felt/50 ㎖ and 56.4% lipid content, and 73.9% triglyceride in the neutral lipid.
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